RE: Slides for immunofluorescence staining

From:"Cummings, Lori Ann"

It is best to stay away from any protein based adhesives as they produce
high background on the slide and interfere with the reaction you are trying
to achieve.  I strongly recommend charged slides (superfrost plus) or
silanized slides for any and all immunofluorescent, immunohistochemical or
in situ hybridization reactions.
Lori A. Cummings 
Clinical Coordinator, Anatomic Pathology 
University of Illinois Medical Center @ Chicago 
office: 312.413.1607 
fax: 312.413.1346 
page id: 6315 

-----Original Message-----
From: []
Sent: Wednesday, June 04, 2003 12:17 PM
Subject: Slides for immunofluoresence staining

I would like to hear from others who do IF staining.  What type of slides do
you use?  We've been using plain slides which we coat with egg albumin, and
store in the Revco until ready to use.  Can poly-l-lysine slides or
superfrost plus slides be used?  

Ellen Yee 

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