Mohammad & Denise, we use both 'Mollifex" by BDH or make 20% KOH, check
the nail every so often to if it is soft enough, it may take an hour or
more.
Mike
 
Michael Konopka
Laboratory Manager
Skin & Cancer Foundation Australia
 
-----Original Message-----
From: Histology [mailto:histo@skm.org.pk]
Sent: Thursday, 5 June 2003 11:13 PM
To: Mike Konopka
Subject: RE: Nail by paraffin section?
 
Dear Michael Konopka 
Thanks for your e-mail. Please tell me what softener method are you
using. 
Again thank you very much. 
 Muhammad Tahseen 
Histology Supervisor 
SKMCH&RC 
  
-----Original Message----- 
From:   Mike Konopka [SMTP:mkonopka@scfa.edu.au] 
Sent:   Thursday, June 05, 2003 3:46 AM 
To:     Histonet (E-mail) 
Subject:        RE: Nail by paraffin section? 
Muhammad, you can use any routine fungal stain, we use PAS, you have 
trouble keeping the section on even with PAS let along a silver 
technique. We coat our slides with 'Aquadhere' which is a wood glue, 
this works in the majority of cases. 
PS soften the nail prior to cutting with whichever method you like prior

to cutting. 
Mike 
  
Michael Konopka 
Laboratory Manager 
Skin & Cancer Foundation Australia 
  
-----Original Message----- 
From: Histology [ mailto:histo@skm.org.pk  ] 
Sent: Wednesday, 4 June 2003 10:33 PM 
To: histonet@pathology.swmed.edu 
Subject: Nail by paraffin section? 
  
Can anyone out there in histoland tell me what the procedure is for 
detection of fungal infection of nail by paraffin section? Thanks 
Muhammad Tahseen 
Histology Supervisor 
SKMCH&RC