Muhammad, you can use any routine fungal stain, we use PAS, you have
trouble keeping the section on even with PAS let along a silver
technique. We coat our slides with 'Aquadhere' which is a wood glue,
this works in the majority of cases.
PS soften the nail prior to cutting with whichever method you like prior
to cutting.
Mike
 
Michael Konopka
Laboratory Manager
Skin & Cancer Foundation Australia
 
-----Original Message-----
From: Histology [mailto:histo@skm.org.pk]
Sent: Wednesday, 4 June 2003 10:33 PM
To: histonet@pathology.swmed.edu
Subject: Nail by paraffin section?
 
Can anyone out there in histoland tell me what the procedure is for
detection of fungal infection of nail by paraffin section? Thanks 
Muhammad Tahseen
Histology Supervisor
SKMCH&RC