I don't retrieve frozen sections at all.  Since their proteins haven't been
cross-linked by formalin, antigen retrieval isn't needed like it is when
dealing with a formalin fixed paraffin embedded piece of tissue.
 
Good Luck,
 
Glen Dawson

-----Original Message-----
From: ulso [mailto:whatisdog@ksvcp.com]
Sent: Friday, June 13, 2003 3:53 PM
To: histonet@pathology.swmed.edu
Subject: In need of optimal AR for frozen sections....


Dear All
 
I am a newcomer for ICC, and I need a protocol for frozen sections
regarding antigen retrieval. I am strage for frozen sections! 
 
Would anyone tell me the optimal protocol for antigen retrieval of frozen
sections? I usually use pressure cooker method, and what is the time for
AR, and buffer solution pH 6.0 or pH 10.0, etc....? 
 
Thanks in advance
 
Ul Soo Choi
DVM, Ph. D. course student
Seoul, Korea