RE: In need of optimal AR for frozen sections....
Dear Ul Soo Choi,
When handling cryostat sections from a fresh frozen tissue block you don't
need any antigen retrieval at all. AR on paraffin sections is just done to
break down the cross-links in the tissue section caused by the formaldehyde
fixation. Cryostat sections from fresh frozen tissue blocks needs to be
fixed after sectioning, using pure acetone (10 min), neutral buffered
formalin (5 min), etc. If you choose for the NBF fixation, you don't need
the AR either, because the NBF fixation is far too short to introduce any
cross-linking in the tissue section.
Good luck with staining!
Chris van der Loos
Academical Medical Center
Dept. of Cardiovascular Pathology
Amsterdam - The Netherlands
>Date: 13 Jun 2003 00:00:19 -0500
>Subject: In need of optimal AR for frozen sections....
>I am a newcomer for ICC, and I need a protocol for frozen sections regarding
>antigen retrieval. I am strage for frozen sections!
>Would anyone tell me the optimal protocol for antigen retrieval of frozen
>sections? I usually use pressure cooker method, and what is the time for AR,
>and buffer solution pH 6.0 or pH 10.0, etc....?
>Thanks in advance
>Ul Soo Choi
>DVM, Ph. D. course student
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