RE: In need of optimal AR for frozen sections....

From:Tony Henwood

If the frozen sections have not been fixed in formalin then antigen retrieval is not required (at least for the antigens I am familiar with).

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: ulso []
Sent: Saturday, 14 June 2003 6:53
Subject: In need of optimal AR for frozen sections....

Dear All
I am a newcomer for ICC, and I need a protocol for frozen sections regarding antigen retrieval. I am strage for frozen sections!
Would anyone tell me the optimal protocol for antigen retrieval of frozen sections? I usually use pressure cooker method, and what is the time for AR, and buffer solution pH 6.0 or pH 10.0, etc....?
Thanks in advance
Ul Soo Choi
DVM, Ph. D. course student
Seoul, Korea

This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please
delete it and notify the sender.

Views expressed in this message and any attachments are those
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any
consequential damage resulting from email containing computer

<< Previous Message | Next Message >>