RE: In need of optimal AR for frozen sections....

From:Tony Henwood

If the frozen sections have not been fixed in formalin then antigen retrieval is not required (at least for the antigens I am familiar with).
 

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

 
 
-----Original Message-----
From: ulso [mailto:whatisdog@ksvcp.com]
Sent: Saturday, 14 June 2003 6:53
To: histonet@pathology.swmed.edu
Subject: In need of optimal AR for frozen sections....

Dear All
 
I am a newcomer for ICC, and I need a protocol for frozen sections regarding antigen retrieval. I am strage for frozen sections!
 
Would anyone tell me the optimal protocol for antigen retrieval of frozen sections? I usually use pressure cooker method, and what is the time for AR, and buffer solution pH 6.0 or pH 10.0, etc....?
 
Thanks in advance
 
Ul Soo Choi
DVM, Ph. D. course student
Seoul, Korea


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