RE: IF's and skin

From:Rena Fail

I have thawed and refrozen skin to reorient and to get ri of freeze artifact. We do not roll either the skin or muscle in talc.
Rena Fail
-----Original Message-----
From: []
Sent: Thursday, June 05, 2003 6:57 PM
Subject: IF's and skin

1) Does anyone know if skin that is misoriented, freeze artifact....any other problems once frozen can be thawed and refrozen again?  

2) Would thawing/refreeze affect the IF staining afterwards?  

3) If a skin has freeze artifact and if it can be thawed, does it need to be rolled in talc powder before refreezing?  (Muscle biopsies that are thawed/refrozen are rolled in talc powder to get rid of freeze artifact.)

I'm hoping for some quick, good advice before I address a problem at work.
Thanks to whomever can help.

Deb King, HT (ASCP)

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