RE: IF's and skin
Message
I have
thawed and refrozen skin to reorient and to get ri of freeze artifact. We do not
roll either the skin or muscle in talc.
Rena
Fail
1) Does
anyone know if skin that is misoriented, freeze artifact....any other problems
once frozen can be thawed and refrozen again?
2) Would
thawing/refreeze affect the IF staining afterwards?
3) If a
skin has freeze artifact and if it can be thawed, does it need to be rolled in
talc powder before refreezing? (Muscle biopsies that are thawed/refrozen
are rolled in talc powder to get rid of freeze artifact.)
I'm hoping
for some quick, good advice before I address a problem at work.
Thanks to
whomever can help.
Deb King, HT (ASCP)
Email:
WWmn916@aol.com
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