RE: Daily Digest

From:"Belson, Dr. Alan"

How do I remove my name from the Listserve?

Alan Belson

-----Original Message-----
From: HistoNet Server [mailto:histonet@pathology.swmed.edu]
Sent: Monday, June 02, 2003 1:05 AM
To: HistoNet Server
Subject: Daily Digest



----------------------------------------------------------------------

Date: 1 Jun 2003 18:45:11 -0500
From: Tony Henwood 
Subject: RE: Breast Tissue


Patsy,
Interesting!
But don't tissues pass through alcohols during processing? Since tissues,
these days, are rarely completely fixed in formalin prior to processing,
then ethanol probably has a secondary fixing effect.
 
Just my thoughts,
 
Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) 
Laboratory Manager 
The Children's Hospital at  Westmead, 
Locked Bag 4001, Westmead, 2145, AUSTRALIA. 
Tel: (02) 9845 3306 
Fax: (02) 9845 3318 

http://www.histosearch.com/homepages/TonyHenwood/default.html
  
http://us.geocities.com/tonyhenwoodau/index.html
  

- -----Original Message-----
From: Patsy Ruegg [mailto:pruegg@colobio.com]
Sent: Saturday, 31 May 2003 2:50
To: Histolady710@aol.com; HISTONET@pathology.swmed.edu
Subject: RE: Breast Tissue


To all who hope to do her2neu expression on breast samples.
I attended a conference presented by a pathologist from Phenopath (lg.
reference lab in Seattle (Dr. Gown)) who warned that her2neu expression was
adversely effected by use of alcoholic fixatives.  Phenopath does a lot of
her2neu tests and they are considered experts in this field.
Patsy

- -----Original Message-----
From: Histolady710@aol.com [mailto:Histolady710@aol.com]
Sent: Thursday, May 29, 2003 2:28 PM
To: HISTONET@pathology.swmed.edu
Subject: Breast Tissue


Does anyone out there in histoland have any suggestions for fatty breast
tissue processing?  We are having a terrible time getting some of our breast
tissue fixed well.    Thanks in advance for your responses. 



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Patsy,
Interesting!
But don't tissues pass through alcohols during processing? Since tissues, these days, are rarely completely fixed in formalin prior to processing, then ethanol probably has a secondary fixing effect.
 
Just my thoughts,
 

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http:// www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities. com/tonyhenwoodau/index.html

-----Original Message-----
From: Patsy Ruegg [mailto:pruegg@colobio.com]
Sent: Saturday, 31 May 2003 2:50
To: Histolady710@aol.com; HISTONET@pathology.swmed.edu
Subject: RE: Breast Tissue

To all who hope to do her2neu expression on breast samples.
I attended a conference presented by a pathologist from Phenopath (lg. reference lab in Seattle (Dr. Gown)) who warned that her2neu expression was adversely effected by use of alcoholic fixatives.  Phenopath does a lot of her2neu tests and they are considered experts in this field.
Patsy
-----Original Message-----
From: Histolady710@aol.com [mailto:Histolady710@aol.com]
Sent: Thursday, May 29, 2003 2:28 PM
To: HISTONET@pathology.swmed.edu
Subject: Breast Tissue

Does anyone out there in histoland have any suggestions for fatty breast tissue processing?  We are having a terrible time getting some of our breast tissue fixed well.    Thanks in advance for your responses.


**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please
delete it and notify the sender.

Views expressed in this message and any attachments are those
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any
consequential damage resulting from email containing computer
viruses.
**********************************************************************
- --Boundary_(ID_AaDB27WcSO8vQrgugFBKVg)-- ---------------------------------------------------------------------- Date: 1 Jun 2003 18:45:31 -0500 From: Tony Henwood Subject: RE: Formalin for processors and HPY billing Kevin, We use 70% ethanol to rinse the formalin prior to processing to absolute ethanol. Decreases the salt build up dramatically. I also thought it prudent to do a warm soapy water and hot water rinse prior to processor solution change. Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: (02) 9845 3306 Fax: (02) 9845 3318 http://www.histosearch.com/homepages/TonyHenwood/default.html http://us.geocities.com/tonyhenwoodau/index.html - -----Original Message----- From: kevin williams [mailto:akwilliams75@hotmail.com] Sent: Saturday, 31 May 2003 5:00 To: histonet@pathology.swmed.edu Subject: Formalin for processors and HPY billing Dear All: I am in terested in views on what formalin you use for your processors. I have a Sakura Tissue Tek VIP. We are using Tissue Tek "VIP Formalin" This stops the build up of salts and removes the need to do water rinse. Can anyone tell me how important it is, is regular formalin, with a rinse just as good. I am also interested in optimizing my processing. We have only skin tissue and will be using xylene. What protocols are you all running out there. I am interested to know. My processing is good but there is always room for improvement. Finally on a completely different note. HPY(the immuno stain), can my pathologist bill for it? Can we make money out of it? Sorry about the mercenary nature of the final question, remember the patient. Thanks and have a good weekend. Yours faithfully, A. Kevin Williams. _____ Protect your PC - Click here for McAfee.com VirusScan Online ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This footnote also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ******************* NOTE ******************* There may be important message content contained in the following MIME Information. ******************************************** - ------------------ MIME Information follows ------------------ This message is in MIME format. Since your mail reader does not understand this format, some or all of this message may not be legible. - --Boundary_(ID_F4EH8YnIOBnDL6HoSi8G+g) Content-type: text/plain; charset=iso-8859-1 Content-transfer-encoding: 7BIT <<<<<< See above "Message Body" >>>>>> - --Boundary_(ID_F4EH8YnIOBnDL6HoSi8G+g) Content-type: text/html; charset=iso-8859-1 Content-transfer-encoding: 7BIT
Kevin,
 
We use 70% ethanol to rinse the formalin prior to processing to absolute ethanol. Decreases the salt build up dramatically. I also thought it prudent to do a warm soapy water and hot water rinse prior to processor solution change.
 

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http:// www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities. com/tonyhenwoodau/index.html

-----Original Message-----
From: kevin williams [mailto:akwilliams75@hotmail.com]
Sent: Saturday, 31 May 2003 5:00
To: histonet@pathology.swmed.edu
Subject: Formalin for processors and HPY billing

Dear All:

I am in terested in views on what formalin you use for your processors. I have a Sakura Tissue Tek VIP. We are using Tissue Tek "VIP Formalin" This stops the build up of salts and removes the need to do water rinse. Can anyone tell me how important it is, is regular formalin, with a rinse just as good.  

 I am also interested in optimizing my processing. We have only skin tissue and will be using xylene. What protocols are you all running out there. I am interested to know. My processing is good but there is always room for improvement.

Finally on a completely different note. HPY(the immuno stain), can my pathologist bill for it? Can we make money out of it?

Sorry about the mercenary nature of the final question, remember the patient.

Thanks and have a good weekend.

Yours faithfully,

A. Kevin Williams. 



Protect your PC - Click here for McAfee.com VirusScan Online


**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please
delete it and notify the sender.

Views expressed in this message and any attachments are those
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any
consequential damage resulting from email containing computer
viruses.
**********************************************************************
- --Boundary_(ID_F4EH8YnIOBnDL6HoSi8G+g)-- ---------------------------------------------------------------------- Date: 1 Jun 2003 19:15:53 -0500 From: Banjo Adesuyi Subject: HTL(ASCP) PRACTICAL
   Dear Colleagues,
             ;             Please I will appreciate it, If  you can help me with some of the specimens listed below for my HTL (ASCP) Practical test;
 
     1) Bone - 1.0cm in lenght, to include cortex and hematopoietic marrow.
 
      2) Kidney - 1.0x1.0cm square,  to include cortex and mudella.
 
      3) Thyroid - 1.0x1.0cm square.
 
      4) Cerebellum -1.0x1.0cm square,  to include  grey and white matter.
 
      5) Skeletal muscle -1.0x1.0cm.
 
             ;  You guys are doing great. You are the best.
 
         Adesupo Banjo Adesuyi  BS, HT(ASCP)
             ; Pathology Department,
           Val Verde Regional Medical Center,
           801 N Bedell Avenue,
           Del Rio, TX 78840.


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