RE: Breast Tissue

From:

"Soto, Roxanne"

I have contacted DAKO about this because this is such a great concern for clinical trial purposes. I spoke to the regulatory department at DAKO and I was told that if the protocol is not followed than it is not a reliable test to determine eligibility for herceptin and patients must show proof of an FDA approved test. I am waiting to hear back from them as I explained that we, like many other labs, don't necessarily fix for 18 - 24 hours in formalin.

My question is----how many people use the FDA approved test and how many people use the non-FDA approved her2neu? And are you fixing the tissue for 18 - 24 hours? I know other labs, that not only do not fix the tissue appropriately, but also tweak the antibody or use it on other machines besides the DAKO. So, if anyone out there is histoland has done a study on how the fixation time either offers false positives or false negatives, I would be greatly interested. And if they are being sent out for FISH anyway, how much does it matter? (This is the question of the Pathologist).

Roxanne

-----Original Message-----
From: Tony Henwood [mailto:AnthonyH@chw.edu.au]
Sent: Sunday, June 01, 2003 6:37 PM
To: 'Patsy Ruegg'; Histolady710@aol.com; HISTONET@pathology.swmed.edu
Subject: RE: Breast Tissue

Patsy,

Interesting!

But don't tissues pass through alcohols during processing? Since tissues, these days, are rarely completely fixed in formalin prior to processing, then ethanol probably has a secondary fixing effect.

Just my thoughts,

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html

-----Original Message-----
From: Patsy Ruegg [mailto:pruegg@colobio.com]
Sent: Saturday, 31 May 2003 2:50
To: Histolady710@aol.com; HISTONET@pathology.swmed.edu
Subject: RE: Breast Tissue

To all who hope to do her2neu expression on breast samples.

I attended a conference presented by a pathologist from Phenopath (lg. reference lab in Seattle (Dr. Gown)) who warned that her2neu expression was adversely effected by use of alcoholic fixatives. Phenopath does a lot of her2neu tests and they are considered experts in this field.

Patsy

-----Original Message-----
From: Histolady710@aol.com [mailto:Histolady710@aol.com]
Sent: Thursday, May 29, 2003 2:28 PM
To: HISTONET@pathology.swmed.edu
Subject: Breast Tissue

Does anyone out there in histoland have any suggestions for fatty breast tissue processing? We are having a terrible time getting some of our breast tissue fixed well. Thanks in advance for your responses.
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