We had this problem, and, after validation, switched over all antibodies
that require high pH(Tris)) to an EDTA buffer. This resolved our problems
and the reactions are every bit as crisp and intense
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972
From: Mitchell, Jeannette M. [mailto:Jeannette.Mitchell@vtmednet.org]
Sent: Thursday, June 05, 2003 7:07 AM
Is there anyone out there that has solved the problem of tissues falling off
when using high pH target retrieval?? Our pathologist is getting quite
upset with repeats to put it mildly.
Thanks for your help
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