OCT inclusion

From:Nancy.Walker@sanofi-synthelabo.com

We do it by putting samples on a flash freeze block in our Shandon
cryostat, then  pouring little by litte OCT around and on top so as to
prevent the specimen from unfreezing. If you don't have this option on your
cryostat I could suggest doing the OCT inclusion in a really cold place
like on dry ice. We've done specimens (large and small) like this and then
do successful ISH.
good luck.




Nancy Walker
Molecular Biology Scientist

Sanofi-Synthelbo Research
B.P. 37 Labége Innopole
31676 LABEGE CEDEX FRANCE

nancy.walker@sanofi-synthelabo.com
tel : (33)561004179  fax :(33)561004001






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