OCT inclusion
From: | Nancy.Walker@sanofi-synthelabo.com |
We do it by putting samples on a flash freeze block in our Shandon
cryostat, then pouring little by litte OCT around and on top so as to
prevent the specimen from unfreezing. If you don't have this option on your
cryostat I could suggest doing the OCT inclusion in a really cold place
like on dry ice. We've done specimens (large and small) like this and then
do successful ISH.
good luck.
Nancy Walker
Molecular Biology Scientist
Sanofi-Synthelbo Research
B.P. 37 Labége Innopole
31676 LABEGE CEDEX FRANCE
nancy.walker@sanofi-synthelabo.com
tel : (33)561004179 fax :(33)561004001
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