For making frozen sections, I wonder whether could I pre-freeze isopentane in a -70C fridge,and then snap freeze the specimen in it?
I know commonly people use dry-ice or LN2 to precool isopentane.But I wonder if -70C firdge could subsititute dry-ice for convenience?
BTW,how safe is isopentane? How will it explode?
Jinling Hospital
Nanjing China