In need of optimal AR for frozen sections....


Dear All
I am a newcomer for ICC, and I need a protocol for frozen sections regarding antigen retrieval. I am strage for frozen sections!
Would anyone tell me the optimal protocol for antigen retrieval of frozen sections? I usually use pressure cooker method, and what is the time for AR, and buffer solution pH 6.0 or pH 10.0, etc....?
Thanks in advance
Ul Soo Choi
DVM, Ph. D. course student
Seoul, Korea

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