How could I get better frozen slides?
In our lab,frozen sections from kidney specimens always look poor.I want to
know how other labs do.
Our procedures are as below,
Firstly we get specimens from clinic bedside.
Take them back to our lab in a 4C box.
Immerse them in OCT.
Put directly in -29C cyrostat and wait for them to be frozen.
Use slides in room temperature to stick to those sectioned tissue. And
frozen sections are prepared.
1) Should we use lower temperature when transfering the specimens? Through
lower temperature is very inconvenient.
2) Should we incubate the specimens in sucrose buffer before immerse them in
OCT? How to do it in details?
3) Should we change a cyrostat? (Ours is from Bright and have used it for
about 10 yrs)
Could you kindly give some suggestions? We just wish to have frozen sections
of better quality.Such as could identify basement membrane and glomeruli in
the setions.Now all we could see is a mass of cells.
Thank you very much!
Yichao WU,Ph.D candidate
Research Insititute of Nephrology,Jinling Hospital
Medical School,Nanjing University
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