| From: | SDSU HISTO |
The GMS stain is usually a more difficult stain however
lately it has become even harder.
We have made up all fresh reagents, switched to using an autostainer
with an old kit and a new kit and changed from 1%periodic acid for 1 hour to
using chromic acid. Some of the
fungi stain but not all. This is
also happening with the PAS stain. The collagen in the GMS stains a nice light
gray.
We use the CBG recycler and add acetic acid to our Propar. Would it be possible for this to affect the
stains? We use reverse osmosis
water and to my knowledge the water has always been the same. When doing the stain I get a silver precipitate
if the process goes to long. Before
when this happened the fungi were dark black. Now they are barely stained.
Any suggestions would be appreciated.
Margaret Perry
Lab Tech
Vet Science Histopath
sdsu_histo@sdstate.edu