Thank you.And I would like to ask could I use isopentane in a common fume hood?
Could you kindly tell me how to use isopentane safely and correctly? How do you do in your lab?
Sincerely
Yichao WU
>From: "Scholz, Stephen J."
>To: "yichao wu" ,
>Subject: RE: Is isopentane safe and could it be used in -70C firdge?
>Date: Thu, 5 Jun 2003 08:00:27 -0500
>
>STOP!!!
>
>Do not put isopentane in a fridge or freezer unless it is explosion-proof. If there is any leak in the bottle there is no place for the HIGHLY EXPLOSIVE fumes to go. I strongly suggest that you read Sakura's Histologic Vol. XXXVI, No. 1, May 2003. The burned out lab on the front cover will give you pause.
>
>Stephen J. Scholz HT(ASCP)
>Histology Coordinator
>OSF St. Anthony Medical Center
>
> -----Original Message-----
> From: yichao wu [SMTP:yichaowu@hotmail.com]
> Sent: Thursday, June 05, 2003 12:25 AM
> To: histonet@pathology.swmed.edu
> Subject: Is isopentane safe and could it be used in -70C firdge?
>
> For making frozen sections, I wonder whether could I pre-freeze isopentane in a -70C fridge,and then snap freeze the specimen in it?
>
> I know commonly people use dry-ice or LN2 to precool isopentane.But I wonder if -70C firdge could subsititute dry-ice for convenience?
>
> BTW,how safe is isopentane? How will it explode?
>
> Yichao WU (yichaowu@hotmail.com)
> Jinling Hospital
> Nanjing China
>
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