Combined Immunohistochemistry with FISH

From:LD Ridley

Dear all,
         Hi, I am currently carrying out my project utilizing immunohistochemistry on FFPE tissues but I am making an enquiry to see if anyone has had any experiencing of combining immunohistochemistry and Fluorescent in situ Hybridization (FISH) on the same (FFPE) tissue slide. I am not sure even if this can be done but I would be really interested in hearing from anyone who has maybe attempted this with success. I am interested to know what detection methods you used for immnuohistochemistry which still allowed visulisation of the fluorescent probes using FISH.

Thanks in advance for your help
Lee Ridley

Birmingham Women's Hospital
Reproductive & Child Health
Academic Unit 3RD Floor
United Kingdom
B15 2TG

Tel: +44 (0)121 6074754

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