Combined Immunohistochemistry with FISH
Hi, I am currently carrying out my project utilizing immunohistochemistry on FFPE tissues but I am making an enquiry to see if anyone has had any experiencing of combining immunohistochemistry and Fluorescent in situ Hybridization (FISH) on the same (FFPE) tissue slide. I am not sure even if this can be done but I would be really interested in hearing from anyone who has maybe attempted this with success. I am interested to know what detection methods you used for immnuohistochemistry which still allowed visulisation of the fluorescent probes using FISH.
Thanks in advance for your help
Birmingham Women's Hospital
Reproductive & Child Health
Academic Unit 3RD Floor
Tel: +44 (0)121 6074754
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