osmium tetroxide

From:Anne Undersander

We are working with researchers who would like to do EM and paraffin embedding on the same piece of tissue.  We will receive the tissue following the EM procedure.  Is there any risk of toxicity from the osmium during decalcification in EDTA, processing, embedding, sectioning, or staining?  If so, is there a way to remove the osmium or make it non-toxic for these procedures?
Anne Undersander HT(ASCP)
Vet. Med./Animal Science
University of Minnesota

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