I routinely section my specimens at -20. Is it neccassary to keep the 
cryostat at -20 when it isnt being used? There is some concern that the 
heating and cooling could causing it to wear out the system or damage the 
microtome? Do people shut off their cryostats if they aren't going to be 
using them for an extended period of time? the weekend? overnight?... 
anyways might seem like a silly question but thanks for any help.
Jason.

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