Hi Histonetters,

I have a few questions about cryostat sectioning.  

In what situations is a cryostat better than regular paraffin processing, and why? 

How do you get cryostat sections to stick to a slide for staining?

Do you need to fix tissue for cryostat in paraformaldhyde/sucrose solution?  Can you section fresh/frozen tissue?

 

Thanks in advance,

 

Jocelyn Torcolini

Electron Microscopy Facility

1 Frear South Building

University Park , Pa 16802

 

Phone (814) 865-0212

www.lsc.psu.edu