From:Jocelyn Torcolini

Hi Histonetters,

I have a few questions about cryostat sectioning.  

In what situations is a cryostat better than regular paraffin processing, and why? 

How do you get cryostat sections to stick to a slide for staining?

Do you need to fix tissue for cryostat in paraformaldhyde/sucrose solution?  Can you section fresh/frozen tissue?


Thanks in advance,


Jocelyn Torcolini

Electron Microscopy Facility

1 Frear South Building

University Park , Pa 16802


Phone (814) 865-0212


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