Wholemoutn beta-gal staining on mouse embryos


I am doing wholemount beta gal stainings on mouse embryos (on a 
LacZ-knock-in mice). The well-known problem is the penetrance of the 
stain from 10.5 dpc onwards. I have been wondering if someone tried to 
skin embryos (maybe a quick scalding would not kill b-gal?) or devised 
some other trick to get the penetrance better.
Staining on the frozen sections works but paraffin sections are again a 
problem since PF and paraffin embedding seem to kill b-gal.

Any pointers would be greatly appreciated since my googling around did 
not produce any decent result.



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