WT1 immunostaining


Has anyone used the WT1 antibody (Santa Cruz) in mouse tissue with 
any success?
The tissue's 4% paraformaldehyde fixed. 
Was the heat retrieval method used?
Any suggestions would be appreciated.
Debi Turner
Medical University of SC
Dept. of Cell Biology/Anatomy
171 Ashley Avenue BSB 641
Charleston, SC 29425
(843) 792-1544

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