Technique needed for distinguishing CaCO3 and bone

From:Joseph.Daniel@colorado.edu



I have several slices of bone with calcium carbonate deposited on them. I am 
searching for a good way to readily distinguish between the original bone and 
the deposited calcium carbonate. I can usually do so by visual inspection, but 
the differences are too subtle for my image analysis software to pick up. My 
goal is to be able to quantitatively determine the amount of deposition on the 
bone. If someone knows a stain or some treatment that I might be able to do 
that will help bring out the differences between the calcium carbonate and the 
bone, I will be most appreciative.

Thanks for your help.

Joe Daniel
Grad student
Museum and Field studies Program
University of Colorado at Boulder



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