I do everything you've done with one exception. I recommend wrapping
each slide individually in aluminum foil, much like a stick of chewing
gum, before placing into the slide box. works great and avoids the
problem you cited.



Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974

>>> jason madore  06/25/03 01:48PM >>>
Any suggestions for storing frozen sections? I made many sections ahead
of 
time to avoid multiple freeze thaw cycles of my blocks and the
consequential 
degradation of RNA from thawing. However the sections are developing
ice 
crystals covering the slides, not sure if the ice will harm the
sections or 
the RNA. I have them in a microslide box, in a tuperware container at
-80 
degrees. I recently added some drierite to the tuperware container, 
hopefully that will help. What do other people do to store frozen
slides?

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