I suspect you are right Joey, if your samples spend too long in fixative 
it will be difficult to retrieve immunoreactivity after decalcification. 
With mouse bones we restrict paraformaldehyde fixation to 48hours and then 
decalcify. We have recently discovered Immunocal decalcifier which gives rapid decalcification and enables subsequent 
immunocytochemistry to be carried out. The supplier of Immunocal in Europe 
is   QUARTETT IMMUNODIAGNOSTICA & BIOTECHNOLOGIE
                                                                BERLIN,
                                                                GERMANY.

                Telephone   ++49  (0) 30765 925 0
                  Contact :~    Jurgen Gorzyza

                                        Regards,
                                                    Tony
Histopathology Group
Asthma Biology Department.
RIRP CEDD.
GlaxoSmithKline Medicines Research Centre,
Gunnelswood Road,
STEVENAGE,
Hertfordshire.
  SG1 2NY
tel.          +44 (0)1438 764117
fax.         +44 (0)1438 764782
email.    Tony.J.Savage@gsk.com
mobile    +44 07753609835





"Joey Shepherd" 

02-Jul-2003 15:00

 
 

        To:     histonet

        cc: 
        Subject:        cold decalc

Hi everyone
Does anyone else out there use EDTA decalcifcation at 4'C? Its been 
reccomended to me for good IHC results. If so what strength/recipe do 
you use and how long does it take? I'm currently using a 20% EDTA 
solution at room temperature on mouse hind limbs - it seems to take 
forever and my antigen retrieval for IHC is pretty bad (although I 
strongly suspect that's due to very long periods spent floating in 
formalin - many of these are archived samples!).
Thanks,

Joey