Re: cold decalc
I suspect you are right Joey, if your samples spend too long in fixative
it will be difficult to retrieve immunoreactivity after decalcification.
With mouse bones we restrict paraformaldehyde fixation to 48hours and then
decalcify. We have recently discovered Immunocal decalcifier which gives rapid decalcification and enables subsequent
immunocytochemistry to be carried out. The supplier of Immunocal in Europe
is QUARTETT IMMUNODIAGNOSTICA & BIOTECHNOLOGIE
Telephone ++49 (0) 30765 925 0
Contact :~ Jurgen Gorzyza
Asthma Biology Department.
GlaxoSmithKline Medicines Research Centre,
tel. +44 (0)1438 764117
fax. +44 (0)1438 764782
mobile +44 07753609835
Subject: cold decalc
Does anyone else out there use EDTA decalcifcation at 4'C? Its been
reccomended to me for good IHC results. If so what strength/recipe do
you use and how long does it take? I'm currently using a 20% EDTA
solution at room temperature on mouse hind limbs - it seems to take
forever and my antigen retrieval for IHC is pretty bad (although I
strongly suspect that's due to very long periods spent floating in
formalin - many of these are archived samples!).
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