I have routinely cut at 3 or 4 microns, however, some spirochetes are longer 
than that and you may only be staining pieces of them. not a problem if you 
realize thats what you're looking at.
Tom Truscott USDA-ARS Pullman, Wa

>From: "Carpenter, Judith A." 
>To: NSH 
>Subject: bug stains
>Date: Mon, 23 Jun 2003 08:35:47 -0400
>
>Does anyone cut their bacterial stains at a certain micron ?
>Are there any possible problems with cutting them at 4 micron ?
>i.e. light staining ?
>thanks !
>Jude
>Jude Carpenter, BS, HTL(ASCP)
>Chief Technologist
>FAHC Histopathology
>111 Colchester Ave.
>Burlington, VT  05401
>(802)847-5116
>FAX(802)847-3509
>jude.carpenter@vtmednet.org
>
>
>

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