I think you have overprocessed the specimen. Try to shortend you time in the processor. Take away the 90% alk. 16 hours for small lymphnodes are to long time. Overprocessed specimens are difficoult to cut. Use ice water bath to get the water back in the tissue. First you trim your block the put the in icewater then you take your sections.

Good luck,

Eva Alstr#246#mer

Histolab Products AB

Sweden

 

 

-----Ursprungligt meddelande-----
Fr#229#n: Innocent Mosha [mailto:innopgp01@yahoo.co.uk]
Skickat: den 17 juni 2003 23:16
Till: histonet@pathology.swmed.edu
#196#mne: How Can I Made Thin Sections For H&E From Lymphnode tissue?

Dear fellows!

I`m really experiencing difficulties in making thin section (5microns) for H&E from lymphnode tissue. Lymphnode tissue are usually prompt/immediately fixed in buffered formalin (pH 7.2) for 12 hours before runned in a automated machine for 16 hours. In this machine tissue goes through and agitated in different baths; starting with 70% alcohol (two changes), then 90%, 95% and absolute alcohols (all these in two changes). Then two changes of Xylene follows before embeded in paraffin wax.

The quality of H&E sections of other tissue is good, but with lymphnode the section are thick/poor quality and impose difficulties in making diagnosis.

I appreciate your opinion/advice, so as I can improve the quality of sections for well fare of patients in this part of the world.

 

Thanking you in advance.

 

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