Re: SILVER STAIN PROBLEMS
Do you sub your slides or use positive charged slides? Are you using gelatin in your bath? Are you using reagents from a different vendor? Are these paraffin sections
or fresh frozen on a sliding micotome? Are you mounting you sections and letting
dry completely!!!??? Silver stains are very finicky, is it possible your silver got contaminated? If the ammoniacal solution is done improperly, the siver stain won't look right. But what your desribing may be as simple as letting slides dry longer. If you
get a chance to call the SUN HEALTH RESEARCH INSTITUTE, and talk to Lucia
Sue histology Supervisor they do a modified BIELSCHOWSKY that works consi-
stantly well.. (623) 876-5458
Good Luck
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