Re: Immuno-bed kit
It may be that your specimen is too large. I could be wrong on this. But usually with plastics, the smaller the better. Try a piece that will fit into a JB-4 mold and see if that polymerizes ok. I'm sure some of the plastic experts like Gail will have a good reply.
PS to Cliff who said "your poll in (is?) completely skewed so the results are meaningless. Reminds me of some recent presidential elections. Jeesh, I hope my opinion isn't meaningless... I was told just this week that it's ok to be a histotech. ;)
Sarah Jones HT(ASCP)
Dept. of Vet. Anatomy & Public Health
Texas A&M University
College Station, TX 77843-4458
>>> Joe Moore 06/27/03 01:24PM >>>
We have been experiencing problems trying to use immuno-bed kit from
Polysciences. The tissue is a half section of a rat testis about 1 inch by
1/2 inch. The protocol we have been using is as follows:
Perfusion fixation with 4% paraformaldehyde in 0.05M PBS (pH 7.4) followed
by dehydration in 50, 70, 95, 100x2 EtoH. Infiltration was done with an
"infiltration" solution contained in the kit. Blocks were then polymerized
under vacuum at 4 degrees.
The tissue center shows incomplete polymerization even though we extended
the polymerization times up to twice the maximum recommended and the resin
itself is brittle. Polymerization of blank blocks at room temperature
worked fine and were easily sectioned. Does anyone have any experiences
using the immuno-bed and can shed some light on this problem?
<< Previous Message | Next Message >>