Here you are again.

 

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

-----Original Message-----
From: Sebree Linda A.
Sent: Wednesday, June 18, 2003 11:57 AM
To: 'Innocent Mosha'
Subject: RE: How Can I Made Thin Sections For H&E From Lymphnode tissue?

Then if you know the tissue is well processed, I'd follow the advice you've been receiving from some of the other histonetters:  cutting at 3 or 4 microns with a very sharp blade and the correct cutting angle.  Sometimes soaking the faced block in water, buffer or a weak solution of ammonium hydroxide will help hydrate the tissue if it tends to be very friable.  Other than these recommendations, I'd say it just takes practice to develop a very smooth cutting action to produce evenly thin sections.

 

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

-----Original Message-----
From: Innocent Mosha [mailto:innopgp01@yahoo.co.uk]
Sent: Wednesday, June 18, 2003 11:50 AM
To: Sebree Linda A.
Subject: RE: How Can I Made Thin Sections For H&E From Lymphnode tissue?

Yes! clinicians usually bisect the lymphnodes unless they are very small. Sometimes I leave the specimens in fluid for more than 24 hours! Also I always make about 3mm thick after receiving the tissue from clinics/theater/wards and allows the tissue to stay in fluid for extra 3hours.

"Sebree Linda A." <la.sebree@hosp.wisc.edu> wrote:

Are the lymph nodes bisected to allow better penetration of fluids?

 

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

-----Original Message-----
From: Innocent Mosha [mailto:innopgp01@yahoo.co.uk]
Sent: Tuesday, June 17, 2003 4:16 PM
To: histonet@pathology.swmed.edu
Subject: How Can I Made Thin Sections For H&E From Lymphnode tissue?

Dear fellows!

I`m really experiencing difficulties in making thin section (5microns) for H&E from lymphnode tissue. Lymphnode tissue are usually prompt/immediately fixed in buffered formalin (pH 7.2) for 12 hours before runned in a automated machine for 16 hours. In this machine tissue goes through and agitated in different baths; starting with 70% alcohol (two changes), then 90%, 95% and absolute alcohols (all these in two changes). Then two changes of Xylene follows before embeded in paraffin wax.

The quality of H&E sections of other tissue is good, but with lymphnode the section are thick/poor quality and impose difficulties in making diagnosis.

I appreciate your opinion/advice, so as I can improve the quality of sections for well fare of patients in this part of the world.

 

Thanking you in advance.

 

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