Re: Dirty Background on GMS Stain

From:Claye Clyatt

You might try using a 60 degree waterbath.  The solution will reach the desirable temperature much faster in a waterbath than in an air incubator, which will produce less precipitation.  You may also find that your staining time will be less.


Claye Clyatt
Chief Histotechnologist
Department of Pathology 
Room #BF119
Medical College of Georgia
Augusta, Ga 30912
office   (706) 721-3630
pager  (706) 721-7243-1132

>>> "Scott, Allison D"  06/17/03 10:38AM >>>
Can anyone make a suggestion on how to eliminate the diry background that
forms on the slide when doing a GMS.  We use a 60 degree oven for the GMS.
The silver is preheated in the oven before putting the slides in.  The
silver is filtered prior to preheating, and we use 1% periodic acid instead
of 5% chromic acid, also we use plus slides for the sections.   Any
suggestions would be appreciated.  Thanks in advance.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital 
Houston, Texas

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