Re: Dirty Background on GMS Stain

From:Claye Clyatt

You might try using a 60 degree waterbath.  The solution will reach the desirable temperature much faster in a waterbath than in an air incubator, which will produce less precipitation.  You may also find that your staining time will be less.

Claye

Claye Clyatt
Chief Histotechnologist
Department of Pathology 
Room #BF119
Medical College of Georgia
Augusta, Ga 30912
office   (706) 721-3630
pager  (706) 721-7243-1132
e-mail: cclyatt@mail.mcg.edu


>>> "Scott, Allison D"  06/17/03 10:38AM >>>
Can anyone make a suggestion on how to eliminate the diry background that
forms on the slide when doing a GMS.  We use a 60 degree oven for the GMS.
The silver is preheated in the oven before putting the slides in.  The
silver is filtered prior to preheating, and we use 1% periodic acid instead
of 5% chromic acid, also we use plus slides for the sections.   Any
suggestions would be appreciated.  Thanks in advance.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital 
Houston, Texas





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