RE: secondary antibody after ABC incubation?
If your ABC had nothing to bind to, no harm done.
Rehydrate the slides to PBS washing all very, very
well. Apply the secondary and repeat the ABC. As long
as you have a known positive control, you will be
Institute for Cancer Prevention
Valhalla, New York
--- 96464771 wrote:
> Hey everybody,
> I'm hoping you can help me. Does anyone know if it
> is possible to incubate
> brain sections in a secondary antibody after they
> have been mistakenly
> incubated in tertiary avidin-biotin complex
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