Hi,
If your ABC had nothing to bind to, no harm done. 
Rehydrate the slides to PBS washing all very, very
well. Apply the secondary and repeat the ABC.  As long
as you have a known positive control, you will be
fine.

Vikki Baker
Institute for Cancer Prevention
Valhalla, New York
--- 96464771  wrote:
> Hey everybody,
> 
> I'm hoping you can help me.  Does anyone know if it
> is possible to incubate 
> brain sections in a secondary antibody after they
> have been mistakenly 
> incubated in tertiary avidin-biotin complex
> overnight.
> 
> L.Garrett
> 
> 
> 


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