RE: secondary antibody after ABC incubation?

From:"Wright, Barbara (DNAX)"

L. Garret,

If your primary was not conjugated with biotin then the avidin-biotin
complex did not bind to anything in your tissue.  Rinse your slides well in
PBS (TBS) and proceed with your secondary antibody.

Barbara Wright
Scientist II
901 California Avenue
Palo Alto, CA 94304-1104

-----Original Message-----
From: 96464771 [mailto:LILLIAN.GARRETT@NUIGALWAY.IE]
Sent: Tuesday, June 17, 2003 3:12 AM
Subject: RE: secondary antibody after ABC incubation?

Hey everybody,

I'm hoping you can help me.  Does anyone know if it is possible to incubate 
brain sections in a secondary antibody after they have been mistakenly 
incubated in tertiary avidin-biotin complex overnight.


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