RE: safe Gram's stain for tissue

From:Tony Henwood

RE: safe Gram's stain for tissue

Try decolourising in acetone, and counterstaining in Twort's solution (a mix of fast green and neutral red) or even weak neutral red or acid fuschin

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: []
Sent: Thursday, 26 June 2003 3:26
Subject: safe Gram's stain for tissue

We need a Gram's staining method that does not use picric acid or ether.
The safety folks have a real problem with storing either of these
chemcals. I have used engine starting fluid in a spray can for ether. It
is safe to store in a pressurized can, but the picric acid is still a
problem. I am sure others have already solved this problem.

Douglas Gregg
Plum Island Animal Disease Center

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