| From: | Tony Henwood |
Try decolourising in acetone, and counterstaining in Twort's solution (a mix of fast green and neutral red) or even weak neutral red or acid fuschin
Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html
-----Original Message-----
From: d.gregg@juno.com [mailto:d.gregg@juno.com]
Sent: Thursday, 26 June 2003 3:26
To: histonet@pathology.swmed.edu
Subject: safe Gram's stain for tissue
We need a Gram's staining method that does not use picric acid or ether.
The safety folks have a real problem with storing either of these
chemcals. I have used engine starting fluid in a spray can for ether. It
is safe to store in a pressurized can, but the picric acid is still a
problem. I am sure others have already solved this problem.
Douglas Gregg
Plum Island Animal Disease Center
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