I use a 10% EDTA solution and decalcification takes about 2 weeks at Room
temperature. If your specimens are that large, maybe you should use a
little heat, like 40 to 50 degrees C to speed up the decalicification.
Or use a more aggressive decal like a formic acid based one. Several are
commercially available. Hope this helps.
> From: Driessen, L.
> Sent: Monday, June 30, 2003 12:18 AM
> To: HistoNet Server; Myri37@aol.com
> Subject: Re: decalcification
> Hello Myriam,
> I'm not familiar with your problem, but I was very suprised to see that
> you decalcify your samples for three month. It doesnt take me that long.
> Perhaps I use a much stronger EDTA-solution (25%) than you. Maybe you can
> try mine.
> - 500 gram EDTA
> - 400 ml distilled water.
> - Heat till booiling
> - Add 1600 ml distilled water
> - Add 40 gram NaOH.
> - Solution becomes clear after a while.
> - Let it cool down to roomtemperature
> - Bring the pH to 7,4 with a NaOH
> Decalcify at roomtemperature while stirring.
> Good luck!
<< Previous Message | Next Message >>