RE: Wholemoutn beta-gal staining on mouse embryos

From:"Favara, Cynthia (NIH/NIAID)"

I have been doing B-gal staining on FFPE tissue for years with no problems.
If this would be helpful let me know.

Cynthia Favara

-----Original Message-----
From: przemko []
Sent: Tuesday, June 24, 2003 6:36 AM
Subject: Wholemoutn beta-gal staining on mouse embryos

I am doing wholemount beta gal stainings on mouse embryos (on a 
LacZ-knock-in mice). The well-known problem is the penetrance of the 
stain from 10.5 dpc onwards. I have been wondering if someone tried to 
skin embryos (maybe a quick scalding would not kill b-gal?) or devised 
some other trick to get the penetrance better.
Staining on the frozen sections works but paraffin sections are again a 
problem since PF and paraffin embedding seem to kill b-gal.

Any pointers would be greatly appreciated since my googling around did 
not produce any decent result.



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