RE: Wholemoutn beta-gal staining on mouse embryos
|From:||"Favara, Cynthia (NIH/NIAID)" |
I have been doing B-gal staining on FFPE tissue for years with no problems.
If this would be helpful let me know.
From: przemko [mailto:email@example.com]
Sent: Tuesday, June 24, 2003 6:36 AM
Subject: Wholemoutn beta-gal staining on mouse embryos
I am doing wholemount beta gal stainings on mouse embryos (on a
LacZ-knock-in mice). The well-known problem is the penetrance of the
stain from 10.5 dpc onwards. I have been wondering if someone tried to
skin embryos (maybe a quick scalding would not kill b-gal?) or devised
some other trick to get the penetrance better.
Staining on the frozen sections works but paraffin sections are again a
problem since PF and paraffin embedding seem to kill b-gal.
Any pointers would be greatly appreciated since my googling around did
not produce any decent result.
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