RE: PCR and microtomy

From:"Favara, Cynthia (NIH/NIAID)"

I wipe things down with RNAse ZAP before starting. Change blades between specimens. Wear gloves. That's seems to work well here.
Good luck,

Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

-----Original Message-----
From: apagan []
Sent: Friday, June 20, 2003 9:42 AM
Subject: PCR and microtomy

We have been asked to cut 20 micron sections from paraffin blocks for PCR.  My question is how do others clean/decontaminate the microtome, blades, etc that are used.  Do you also sterilize any forceps or other instruments used, and does the tech wear sterile gloves?  Since the tissue was not treated specially up to this point (we did not know of the pcr request) does it really matter that everything is sterile now?  We did purchase DNAase and RNAase free vials to put the ribbons in.

Audrey Pagan

Laboratory Manager

Lakewood Pathology Associates

1200 River Ave.  Suite 10E

Lakewood, NJ   08701

732-901-7575  ext. 102



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