Dear Brooke,

If your tissue has been processed using alcohols or zylenes the fat will
probably not survived.  Unless you fixed/postfixed your tissues in Osmium
Tetroxide before processing.  This method is not commonly used becaus of the
extreme hazards involved of course.

Donald G. Awbrey, HT (ASCP), QIHC
Electron Microscopy / Image Analysis
817-878-5647
donaldawbrey@texashealth.org 


-----Original Message-----
From: Brooke McCale [mailto:brm3073@hotmail.com]
Sent: Tuesday, July 01, 2003 3:35 PM
To: histonet@pathology.swmed.edu
Subject: OIL RED O HELP


HELP

Is there a technique for Oil Red O or Sudan Black on paraffin sections which

can be done quickly (a couple of hours)?  Thanks

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