RE: MYELIN STAIN
I cut my brain sections at 8um. Mount on any type of slide. Let them air
dry until they turn white, then the 60 degree C oven for one hour. Rarely
will sections fall off for LFB or LFB-PAS.
> ----------
> From: IHC World
> Sent: Wednesday, July 2, 2003 7:36 PM
> To: Maliniak, Richard; histonet@pathology.swmed.edu
> Subject: Re: MYELIN STAIN
>
> I always use gelatin coated slides to mount brain tissue sections. I have
> been cutting 50 um paraffin sections and mounted on getatin coated slides
> stained with LFB for myelin and worked very well. However, sections
> falling off slides may have something to do with fixation as well.
>
> Richard
> _____________________
> IHC World
> Email: info@ihcworld.com
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>
>
> ----- Original Message -----
> From: Maliniak, Richard
> To: histonet@pathology.swmed.edu
> Sent: Wednesday, July 02, 2003 1:38 PM
> Subject: MYELIN STAIN
>
>
> We have not been able to do a myelin (special stain or immunostain)
> on an autopsy specimen because the brain section keep falling off the
> slides. We have tried all types of slides including charged slides and no
> work. Any suggestions on what we should do? Would anyone like us to send
> them the slides to try?
>
> Rick
>
>
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