RE: MYELIN STAIN

From:"Gehan, Loralee"

I cut my brain sections at 8um.    Mount on any type of slide.  Let them air
dry until they turn white, then the 60 degree C oven for one hour.  Rarely
will sections fall off for LFB or LFB-PAS.  


> ----------
> From: 	IHC World
> Sent: 	Wednesday, July 2, 2003 7:36 PM
> To: 	Maliniak, Richard; histonet@pathology.swmed.edu
> Subject: 	Re: MYELIN STAIN
> 
> I always use gelatin coated slides to mount brain tissue sections. I have
> been cutting 50 um paraffin sections and mounted on getatin coated slides
> stained with LFB for myelin and worked very well. However, sections
> falling off slides may have something to do with fixation as well.
>  
> Richard
> _____________________
> IHC World
> Email: info@ihcworld.com
> Web: www.ihcworld.com
> 
> 
> 	----- Original Message ----- 
> 	From: Maliniak, Richard 
> 	To: histonet@pathology.swmed.edu 
> 	Sent: Wednesday, July 02, 2003 1:38 PM
> 	Subject: MYELIN STAIN
> 
> 
> 	We have not been able to do a myelin (special stain or immunostain)
> on an autopsy specimen because the brain section keep falling off the
> slides.  We have tried all types of slides including charged slides and no
> work.  Any suggestions on what we should do?  Would anyone like us to send
> them the slides to try?
> 
> 	Rick 
> 
> 



<< Previous Message | Next Message >>