RE: How Can I Made Thin Sections ?cork
Dear Joyce,
Can you please explain how you use the cork to clean your
knife.
Regards, Laurie.
At 06:49 PM 06/18/03 -0400, Weems, Joyce wrote:
One thing that I find helpful
is to put the block in the warm water bath for a few seconds, then chill
the block with ice. There is a narrow window to obtain the perfect
section - the one right after the artefact and the one just before you
reach the untreated tissue again.
The way to make your knife edge
last longer is to clean it with a cork just before taking the section. It
is amazing how long the life of the blade can be extended using the cork
from a wine bottle. Works on regular knives you sharpen also.
Hope this helps. It's hard to
believe I'm sitting here in Atlanta, "talking" to someone in
East Africa. Isn't it an amazing world! j
- -----Original Message-----
- From: Innocent Mosha
[mailto:innopgp01@yahoo.co.uk]
- Sent: Wednesday, June 18, 2003 5:47 PM
- To: Fred Underwood
- Cc: histonet@pathology.swmed.edu
- Subject: RE: How Can I Made Thin Sections For H&E From
Lymphnode tissue?
- Thank you Fred!!
- I agreed with you, that too much trimming of block and chill in Ice
bath may give ice artefact on section as well shrinkage artefact. Over or
underfixation could be the factor causing this problem, but why
lymphnodes is the only victim? lymphoid tissue are rather solid, but it
is possible to thin sections from cartilage, brain, liver even bones. A
point of using new sharp knife is a temporary solution, especially for us
poor resourse countries. With old sharpened knife we can make thin
sections from various tissue, why not lymphnodes? Some of the helpful
hints have used previously with little effect!
- I think there might be confounding factors, other than what we have
been discussing!!!!
- Histonetters! do you occasionally face the similar problem? In need
to here much from you!
-
- Innocent
- Postgraduate student,
- University of Dar es Salaam Muhimbili Campus.
- Tanzania East Africa.
- Fred Underwood <funderwood@mcohio.org> wrote:
- I worked with a pathologist who was a stickler about lymph node
sections. He insisted on virtually all nodes being fixed in B5. Your
processing schedule seems OK. I'll throw out a couple of helpful tips.
- 1.) If the block is crowded with nodes, divide it into one or two
more blocks.
- 2.) Be gentle when facing the block. Cutting away too thick amounts
will create a "moth eaten" effect microscopically.
- 3.) A new blade is a must. Although the edge may be too fine.
Condition the blade by cutting sections from a plain block of paraffin.
- 4.) Do not chill the block with freeze spray. This will cause the
tissue to crack within the block.
- 5.) If the nodes are fixed in B5, it is imperative to completely dry
the section of all water before exposing to heat.
- 6.) If the nodes are not brittle, cool them without exposure to
water. They can become somewhat spongy.
- Good Luck,
- Fred
- -----Original Message-----
- From: Innocent Mosha
- Sent: Tuesday, June 17, 2003 5:16 PM
- To:
- Subject: How Can I Made Thin Sections For H&E From Lymphnode
tissue?
- Dear fellows!
- I`m really experiencing difficulties in making thin section
(5microns) for H&E from lymphnode tissue. Lymphnode tissue are
usually prompt/immediately fixed in buffered formalin (pH 7.2) for 12
hours before runned in a automated machine for 16 hours. In this machine
tissue goes through and agitated in different baths; starting with 70%
alcohol (two changes), then 90%, 95% and absolute alcohols (all these in
two changes). Then two changes of Xylene follows before embeded in
paraffin wax.
- The quality of H&E sections of other tissue is good, but with
lymphnode the section are thick/poor quality and impose difficulties in
making diagnosis.
- I appreciate your opinion/advice, so as I can improve the quality of
sections for well fare of patients in this part of the world.
- Thanking you in advance.
- Want to chat instantly with your online friends?#160#Get the FREE
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Mr.Laurie
Reilly
Ph 07 4781 4468
Physiology &
Pharmacology
Fax 07 4779 1526
Aust.Inst.of Tropical Vet.& Animal Sc.
James Cook University
Townsville Qld.
4811
laurie.reilly@jcu.edu.au
Australia.
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