RE: How Can I Made Thin Sections For H&E From Lymphnode tissue?


Thank you Liz!
I`m processing human tissue (lymphnodes), and in several occasions the nodes were bisected unless they are too small.  I grossed in to make about 3mm thick and allows tissue to stay in fixative for extra 3 hours.
Infact, I`ve no problems with other many tissues! this difficulties are experiened on lymphnodes!! Otherwise thank you very much and your advice have been taken!

Elizabeth Chlipala <> wrote:

Your processing cycle is too long.  Cut down the time in each of the stations to 30 minutes to 45 minutes.  Lymph nodes also need to be sectioned thinner than most tissues.  You should try to cut the sections at 2-3 microns.  That way you will have a single cell layer.  I usually rough trim my blocks and then let them soak on ice and water for about an hour.  Are you sectioning animal or human tissue.  If its animal you might be able to cut the processing even more, you also need to make sure the lymph node has been grossed in thin enough.  It should not be thicker than 3 mm.  Its also important with larger lymph nodes to cut them in half, if you submit them whole the capsule around the lymph node could cause problems with processing.  Hope this info helps, if you have any more questions feel free to contact me by phone or e-mail


Good Luck




Elizabeth A. Chlipala, BS, HTL(ASCP)

Premier Histology Laboratory, LLC

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Boulder, Colorado 80308

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-----Original Message-----
From: Innocent Mosha []
Sent: Tuesday, June 17, 2003 2:16 PM
Subject: How Can I Made Thin Sections For H&E From Lymphnode tissue?


Dear fellows!

I`m really experiencing difficulties in making thin section (5microns) for H&E from lymphnode tissue. Lymphnode tissue are usually prompt/immediately fixed in buffered formalin (pH 7.2) for 12 hours before runned in a automated machine for 16 hours. In this machine tissue goes through and agitated in different baths; starting with 70% alcohol (two changes), then 90%, 95% and absolute alcohols (all these in two changes). Then two changes of Xylene follows before embeded in paraffin wax.

The quality of H&E sections of other tissue is good, but with lymphnode the section are thick/poor quality and impose difficulties in making diagnosis.

I appreciate your opinion/advice, so as I can improve the quality of sections for well fare of patients in this part of the world.


Thanking you in advance.



Postgraduate student,

University of Dar es Salaam- Muhimbili Campus.

Tanzania-East Africa.

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