|From:||"Smith, Allen" |
Allen A. Smith, Ph.D.
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161-6695
From: Innocent Mosha [mailto:firstname.lastname@example.org]
Sent: Tuesday, June 17, 2003 5:16 PM
Subject: How Can I Made Thin Sections For H&E From Lymphnode tissue?Dear fellows!I`m really experiencing difficulties in making thin section (5microns) for H&E from lymphnode tissue. Lymphnode tissue are usually prompt/immediately fixed in buffered formalin (pH 7.2) for 12 hours before runned in a automated machine for 16 hours. In this machine tissue goes through and agitated in different baths; starting with 70% alcohol (two changes), then 90%, 95% and absolute alcohols (all these in two changes). Then two changes of Xylene follows before embeded in paraffin wax.The quality of H&E sections of other tissue is good, but with lymphnode the section are thick/poor quality and impose difficulties in making diagnosis.I appreciate your opinion/advice, so as I can improve the quality of sections for well fare of patients in this part of the world.Thanking you in advance.InnocentPostgraduate student,University of Dar es Salaam- Muhimbili Campus.Tanzania-East Africa.
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