Dear Innocent,Jambo!May I suggest that you fix the tissue for longer say 24 hours, lymph node is dense and the buffered formalin may not be penetrating sufficiently, also you do not seem to be post-fixing on your processing schedule so the problem may well be due to under fixation. Also 5 microns is a bit thick for lymphoid tissue, try 3 or 4 microns. Chill your blocks before sectioning to help with the thin sections.What do the other histonetters think?
Best regardsMargaret BlountClinical BiochemistryUniversity of Cambridge-----Original Message-----
From: Innocent Mosha [mailto:firstname.lastname@example.org]
Sent: Tuesday, June 17, 2003 10:16 PM
Subject: How Can I Made Thin Sections For H&E From Lymphnode tissue?Dear fellows!I`m really experiencing difficulties in making thin section (5microns) for H&E from lymphnode tissue. Lymphnode tissue are usually prompt/immediately fixed in buffered formalin (pH 7.2) for 12 hours before runned in a automated machine for 16 hours. In this machine tissue goes through and agitated in different baths; starting with 70% alcohol (two changes), then 90%, 95% and absolute alcohols (all these in two changes). Then two changes of Xylene follows before embeded in paraffin wax.The quality of H&E sections of other tissue is good, but with lymphnode the section are thick/poor quality and impose difficulties in making diagnosis.I appreciate your opinion/advice, so as I can improve the quality of sections for well fare of patients in this part of the world.Thanking you in advance.InnocentPostgraduate student,University of Dar es Salaam- Muhimbili Campus.Tanzania-East Africa.
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