Dear fellows!
I`m really experiencing difficulties in making thin section (5microns)
for H&E from lymphnode tissue. Lymphnode tissue are usually
prompt/immediately fixed in buffered formalin (pH 7.2) for 12 hours before
runned in a automated machine for 16 hours. In this machine tissue goes
through and agitated in different baths; starting with 70% alcohol (two
changes), then 90%, 95% and absolute alcohols (all these in two changes). Then
two changes of Xylene follows before embeded in paraffin wax.
The quality of H&E sections of other tissue is good, but with
lymphnode the section are thick/poor quality and impose difficulties in making
diagnosis.
I appreciate your opinion/advice, so as I can improve the quality of
sections for well fare of patients in this part of the world.
Thanking you in advance.
Innocent
Postgraduate student,
University of Dar es Salaam- Muhimbili Campus.
Tanzania-East Africa.
Want to chat instantly with your online
friends?#160#Get
the FREE Yahoo! Messenger