|From:||Gary Gill |
Biebrich scarlet (ws) -- C.I. No. 26905 0.4 gm
Eosin Y -- C.I. No. 45380 5.0 gm
Phloxine B -- C.I. No. 45410 2.1 gm
95% ethanol 200 mL
Distilled water 800 mL
The weights given are for total dye content. Greater amounts, therefore, must be weighed out to compensate for dye content. Biebrich scarlet is not certified by the Biological Stain Commission, so no dye content will be printed on the label. Acetic acid is not added. Combine the ingredients at room temperature, stir, and filter before using. Staining time is a few dips.
From: kevin williams [mailto:email@example.com]
Sent: Tuesday, June 24, 2003 11:55 AM
Subject: Eosin and beafing it up a bit.Dear All:I am trying to increase my eosin staining on my slides, I have a linistainer and the slides only go into the stain for 10 seconds. So I want to make the solution as strong as I can.Can I just add more dye powder, or do I need to increase the concentraions of the Acetic Acid,How dose Phloxine work, will I be able to get better results?Yours faithfully,A. Kevin Williams.
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