RE: Dirty Background on GMS Stain
How long are you leaving them in silver? Usually once the sections turn a light brown, it's time to take them out of the silver. Also, lower your oven temp a few degrees. This will give you a little more time between light brown and jet black.
From: "Scott, Allison D"
Sent: Tuesday, June 17, 2003 10:38 AM
Subject: Dirty Background on GMS Stain
Can anyone make a suggestion on how to eliminate the diry background that
forms on the slide when doing a GMS. We use a 60 degree oven for the GMS.
The silver is preheated in the oven before putting the slides in. The
silver is filtered prior to preheating, and we use 1% periodic acid instead
of 5% chromic acid, also we use plus slides for the sections. Any
suggestions would be appreciated. Thanks in advance.
Allison Scott HT(ASCP)
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