Martius scarlet blue stain for fibrin (fwd)

From:Sarka Lhotak

Heelo Netters,
	I am reposting this message hoping that this time somebody will
notice it and will be able to help us (John Kiernan, Brian Hewlett???).
Thanks a lot,

---------- Forwarded message ----------
Date: Tue, 24 Jun 2003 15:12:33 -0400 (EDT)
From: Sarka Lhotak 
Subject: Martius scarlet blue stain for fibrin

Hello Netters,
	I am posting this for a friend, Filip Konecny. Please reply either
to the list or directly to him :

According to Buk SJ, Stain Technol. 1984;59(1):1-5, the Martius scarlet
blue trichrome (MSB) stains selectively for fresh, mature and old fibrin
(ranging from orange through red to blue). During the fibrin maturation
its structure is changing by polymerization and crosslinking. Would
anybody know how do the different MSB colours correspond to the actual
stages of fibrin polymerization and crosslinking?
	 In my experiment I use lytic agents in-vivo to partially
dissolve fibrin thrombi. Would the fibrin degradation products be
stained and what colour with the MSB stain? Can one resolve newly
self-assembled lytic fragments?
	What is being stained by MSB so that it can reflect the process of
fibrin maturation?
	Any input is greatly appreciated.

Filip Konecny
Henderson Research Centre
McMaster University
Hamilton, Ontario, Canada

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