Whoops, hit the send button to soon - 

>Doreen,
>
>You did not say how you were doing the staining protocol and what
decolorizer is being used.  I could not find a method specifically named
Hucker Conn (unless it is a purchased kit) but Lillie's book, for a Gram
stain on tissue sections, uses Hucker Conn crystal violet as does Taylors
modification of Brown and Brenn.  

I suspect your decolorizer as the culprit.  

Can you elaborate on exact procedure as Gram stains on tissue sections have
many variations/modifications. 

Thanks 

   


Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
S. 19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

email: gcallis@montana.edu