Cyclin D1
Rita
This is quite a
difficult antigen and requires intense antigen retrieval. We've found a
combination of Pressure cooking in Tris-EDTA pH8.0 followed by 15 seconds in
0.05% Pronase E to give good results with the Novocastra antibody at 1:50. You
can also use a high pH AR solution like Dako's 'High pH Target Rerieval
Solution' which is pH 9.9.
Detection is
PowerVision which is more sensitive than most other systems.
Hope this
helps
Lawrence
Brett
Royal Infirmary,
Edinburgh. UK
_____________________________________________________________
Dear
Histonetters,
I am trying to
get an optimum staining technique for Cyclin D1 on bone
marrow trephines.
Any advice as to what you are doing to make this difficult
stain
reproducibly and reliably accurate? (antibodies, retrieval, fixation,
kits,
timing, etc..) Any good ideas or hints would be much
appreciated.
Many thanks from Down Under!
Rita Broner
Bone
Marrow Transplant Unit
Alfred Hospital
Melbourne, Australia
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