x-gal staining fixation
We have just started doing x-gal staining in our lab on frozen sections. A
researcher brought us b-gal positive control liver tissue to use to work out
the stain. Several of the protocols we looked up including those found in
histonet archives use a prefixation step before freezing the tissue in liquid
nitrogen. We found out that the researcher did not do any prefixation and only
froze the tissue before giving it to us. Does anyone know how important this
step is, and is this possibly the reason why we are not getting any staining???
Thanks for any input!
Pathology Core Facility
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